[scipion-users] Local Resolution estimation(MonoRes and DeepRes) and Local Deblur

[范宏成 <187...@16...>]

Dear Colleagues,
I have some questions about Local resolution estimation and Local Deblur.  Looking forward to your response . Thank you!
1. MonoRes. Can MonoRes estimate the local resolution when I do focused refinement (masked refinement) using relion. I mean there maybe no region  to estimate the noise.  In this condition, I have to use the relion local resolution protocol to get the focused map local resolution. 
2. Local Deblur.  Can Local Deblur support the relion local resolution map or only support MonoRes,Resmap, Blocres?
3. DeepRes. I recently read a paper about the DeepRes and find it very useful for my project.  I didn't find the DeepRes protocol in my scipion. My scipion version is v2.0(2019-04-23) Diocletian in Centos7 system(Binary version).  How can I use this protocol? 
4.DeepRes for membrane protein. In DeepRes paper, It said it is very important to mask out the micelle because DeepRes only trained with proteins.  If I don't mask out the micelle, What bad consequencies will happen? By the way, How to effectively and carefully  mask out the micellce? In my way, I deleted the micelle using particle substraction and run again auto-refinement in relion. In order not to delete the signal from the protein, I used to use the soft mask, So My membrante protein  had a small number of micelles around the TM domain. I'm afraid this residual micelle may cause bad consequencies when using DeepRes. Does someone have a better way to delete the micelle in membrane proteins?


Fan Hongcheng


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范宏成
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m18...@16...
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