[scipion-users] Combining 2 dataset --- coordinates/extraction issue

[Dmitry S. <Sem...@gm...>]

Dear colleagues,

 I have 2 sets of particles from different micrographs that have the same pixel size.

 

I want to join (combine) them and run all together.

 What can be the strategy to do that?

 



What I did

a)     Combined 2 sets of micrographs (with which I worked independently).

Combined 2 sets of particles;

Tried to extract the combo coordinates from the combined micrographs.

Error --- part of them extracted; part of them has wrong coordinates.

 

And even with wrong coordinates if I run the 3d auto refinement I get the error Protocol failed: no path specified.

 

b)    Combined all the micrographs in 1 project.

Run MotionCor + CTF estimation

Somehow import the combined coordinates from a) to this new project.

Re-extract the coordinates.

 

 

So I have a questions:

-       Why a) doesn’t work? Is there a bug?

-       How to export/import the combined coordinates into the new project?

Amy other suggestions?

 

p.s. when/if I succeed with a) with the particle polishing and ctf refinement work? The 2 datasets have different dose rate info.
Thank you!

 

Sincerely,

Dmitry