Re: [scipion-users] K3 tiff format movies

[Carlos O. S. S. <co...@cn...>]

Dear Nora,


the current Scipion model does not allow for several gain images. Have 
you found a situation where this is needed or customary? We understand 
that collecting the gain image takes about 1h, if not more, is that correct?


Kind regards, Carlos Oscar


El 23/09/2019 a las 16:49, Nora Cronin escribió:
> Hi
>
> I am attempting to process K3 "tiff LZW non gain normalised" movies in 
> Scipion.     There is one gain reference file included in each 
> gridsquare directory.       In the import GUI I input the name of the 
> directory /Gridsquare*/Data   and for the Pattern "*_fractions.tiff ".
> Since the name of the gain is different in each Gridsquare - how do I 
> input the gain There is one gain reference for all the movies in that 
> particular grid square    Inputing the one gain reference in the 
> Gridsquare/Data subdirectory did not work - motioncorr did not find 
> the gain reference after the first movie.
>
> Should I use the gain reference directly form DM (the .dm4) file and 
> if so how should I convert it?
>
> Best regards
>
>
> Nora
> Nora Cronin
> LonCEM Facility Manager
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Carlos Oscar Sánchez Sorzano                  e-mail:   co...@cn...
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