Re: [scipion-users] K3 tiff format movies

[Pablo C. <pc...@cn...>]

Hi Nora!


All users I know they are using the dm4 gain and one gain per import. I 
personally do not see the benefit of having a gain per movie/gridsquare. 
Although I might be wrong and it might

be something actually useful to have.


Scipion will do the convertion from dm4 to mrc, so you don't need to 
bother with format conversions.


There is one thing you might want to check, and is the gain orientation, 
so far you need to flip it on Y if your gain is dm4.


For a quick check, make a subset of the movies with one movie an apply 
ply with that movie, motioncor and the the orientation.


If the gain is very subtle, I haven't found a way to verify the 
orientation, in that case I would set "Flip gain reference" to "upside down"



Bear in mind that you need to activate the "Advanced mode" to display 
that field.


All the best, Pablo.


On 23/9/19 16:49, Nora Cronin wrote:
> Hi
>
> I am attempting to process K3 "tiff LZW non gain normalised" movies in 
> Scipion.     There is one gain reference file included in each 
> gridsquare directory.       In the import GUI I input the name of the 
> directory /Gridsquare*/Data   and for the Pattern "*_fractions.tiff ".
> Since the name of the gain is different in each Gridsquare - how do I 
> input the gain There is one gain reference for all the movies in that 
> particular grid square    Inputing the one gain reference in the 
> Gridsquare/Data subdirectory did not work - motioncorr did not find 
> the gain reference after the first movie.
>
> Should I use the gain reference directly form DM (the .dm4) file and 
> if so how should I convert it?
>
> Best regards
>
>
> Nora
> Nora Cronin
> LonCEM Facility Manager
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-- 
Pablo Conesa - *Madrid Scipion <http://scipion.i2pc.es> team*