[scipion-users] K3 tiff format movies

[Nora C. <nor...@cr...>]

Hi

I am attempting to process K3 "tiff LZW non gain normalised" movies in Scipion.     There is one gain reference file included in each gridsquare directory.       In the import GUI I input the name of the directory /Gridsquare*/Data   and for the Pattern "*_fractions.tiff ".
Since the name of the gain is different in each Gridsquare - how do I input the gain There is one gain reference for all the movies in that particular grid square    Inputing the one gain reference in the Gridsquare/Data subdirectory did not work - motioncorr did not find the gain reference after the first movie.

Should I use the gain reference directly form DM (the .dm4) file and if so how should I convert it?

Best regards


Nora

Nora Cronin
LonCEM Facility Manager

The Francis Crick Institute

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