[scipion-users] local resolution

[Arshad, R. <r.a...@ru...>]

Dear All,

I want to improve local resolution of core part of cryo-EM structure, as it
shows flexibility at periphery. I am trying masked 3D classification and
auto-refinement in relion using a mask generated from pdb (added in
'reference mask' tab). Unfortunately, the structure gets distorted at
refinement step. Should I include an extra step between 3D alignment and
auto-refinement?

Moreover, I would also like to try signal subtraction from flexible part of
3D map. Can somebody explain the correct way to do it in scipion?

Thanks in advance.

Best regards,
Rameez Arshad



-- 
Rameez Arshad, PhD student | Electron Microscopy
Groningen Biomolecular Sciences and Biotechnology Institute, University of
Groningen
Nijenborgh 7, 9747 AG, Groningen, The Netherlands
+31 50 363 4378