Hi,
We use FOCUS in our facility to do all the preprocess on-the fly
(motioncor2 ,ctf) and the current version are able to export the dataset
for Relion3 processing.
Best,
On Fri, Feb 22, 2019 at 6:14 PM Jonathan Remis <re...@gm...> wrote:
> Hello Jose Miguel,
>
> We also have interest in using Relion3 from within Scipion.
>
> We currently are trying to use Scipion at our EM facility to monitor data
> as it is collected and typically use Motioncor2 to do the motion
> correction. Many users want to use Relion3 to do their single particle
> reconstructions but because you are not able to import Motioncor2 fixed
> micrographs into Relion3 they need to re-run motion correction from within
> Relion3. It would be ideal if we could give them motion corrected
> micrographs that they could directly use in the image processing program of
> their choice. I was curious if Scipion will or does include access to
> this different multi-core cpu motion correction program? I don't see it
> in the protocols after following the instructions you have posted in the
> link above.
>
> Thank you for your time-
>
> Jonathan_Remis
>
>
>
>
> On Thu, Feb 21, 2019 at 11:22 AM Jose Miguel de la Rosa Trevin <
> del...@gm...> wrote:
>
>> Hi Daniel,
>>
>> I have updated the protocols in Scipion for support for Relion3. It is
>> beta right now,
>> but it has been used here in Stockholm and also a bit in Madrid. We
>> haven't announced
>> yet because we will wait until the next Scipion release (2.0) for that.
>>
>> I wrote some instructions to use it from the current Scipion version
>> (v1.2.1)
>> https://github.com/delarosatrevin/scipion/wiki/Testing-Relion-3-in-Scipion
>> <https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttps-3A__github.com_delarosatrevin_scipion_wiki_Testing-2DRelion-2D3-2Din-2DScipion%26d%3DDwMFaQ%26c%3DyHlS04HhBraes5BQ9ueu5zKhE7rtNXt_d012z2PA6ws%26r%3DD_rs7PhtmkmTUoH64Stdl6OPL5kCXmTvZbb7Ola-aIs%26m%3DWyDyWBGr3WYFt_HcN3ca4g6lI0to6OT4OjaVHX03qM8%26s%3DCRCn6MzQcPw3qmitElhcn8pmM9Kl0tOeUkMbYMZ3j1Y%26e%3D&data=02%7C01%7Celad.binshtein%40vanderbilt.edu%7C0d238b15b65b44df39d108d699241cca%7Cba5a7f39e3be4ab3b45067fa80faecad%7C0%7C0%7C636864777724240833&sdata=kDJA72Yvj7CV9fW1M%2B0oaGmFNVJcZfR423T1DmvTiHY%3D&reserved=0>
>>
>> Please don't hesitate to contact me if you need some help to update the
>> Scipion
>> installation for Relion 3 support.
>>
>> Cheers,
>> Jose Miguel
>>
>>
>> On Thu, Feb 21, 2019 at 8:15 PM Daniel Larsson <dan...@ic...>
>> wrote:
>>
>>> When can we expect to have Relion 3 in Scipion?
>>>
>>> Regards,
>>> Daniel
>>>
>>>
>>>
>>>
>>>
>>>
>>>
>>>
>>>
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--
________________________________
Elad Binshtein, Ph.D.
Cryo-EM specialist - Senior Staff Scientist
Center for Structure Biology (CSB)
MCN Room 1207
Vanderbilt University
Nashville, TN
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