[scipion-users] Use of masks in MonoRes and LocalDebur

[Matthieu B. <bio...@gm...>]

Hi,

Is it possible to use a non-spherical mask to restrict where MonoRes and
LocalDeblur analysis run in a map? I've not seen this option in these
programs but I'm asking anyhow in case there are some suggestions about how
to achieve this. I'm asking because I have over 20 large maps with helical
symmetry that I'd like to postprocess. I like the MonoRes/LocalDeblur
results but the computation takes 2 days for each map on my workstation. If
I could run these programs on a 15% segment of these helical maps (which
contains all the information), then I could just apply helical symmetry to
get the full map and the processing would be much faster.
I suspect that pre-masking the half-maps or giving a mask to MonoRes that
does not include all the signal of the macromolecule in the map would
negatively impact the noise estimation in MonoRes.

Thanks for any advice,

Best,

Matthieu