From: Matthews-Palmer, T. <t.m...@im...> - 2018-03-21 16:02:21
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Dear Scipion-users, I have a situation in Scipion where a dataset of movies was motion-corrected with motioncor2, but aligned movies were not saved (at the time, there was not storage space). Now the processing is at a late stage and I would like to attempt movie refinement & particle polishing, so aligned movie frames are necessary. I cannot restart the motioncorr protocols because they are referenced from subsequent protocols. I could run a new motioncorr protocol. Does anyone know if I will be able to easily transfer co-ordinates and angles from a late-stage set of particles to these new movies to make movie-particles? Will scipion - extract coordinates be able to extract the right co-ordinates from aligned movies? Is there something else I need to bear in mind to handle movie-particles in Scipion? Thanks for any help, All the best Teige |