Re: [scipion-users] 4bit mrc

[Bart <sca...@gm...>]

>
>  For 4-bit, I'd say read into uint8 and do some bit massaging (AND and
shift).
(ideally do it while loading, so that you don't need to effectively
allocate the whole thing two/three times)


And in case of UCSF, the gain reference is a NX*NY float32 image right
after the agard-style extended header.
You can detect its presence by seeing that NSYMBT is exactly that much
(NX*NY*4) larger than NINT and NREAL would suggest.
...as far as I understand it, anyway.



I've been writing a module that reads extended data for all variants that
can come out of our setup, meaning FEI, UCSF, 4-bit, and SerialEM.

While almost no variant marks itself, you can get suprisingly far in
detection.
...though I keep on coming across new and exciting real-world deviations,
so this needs more testing before it's useful to anyone else.


--Bart Alewijnse @NeCEN