Re: [scipion-users] Particle extraction from a dataset filtered based on CTF

[Gregory S. <sha...@gm...>]

Hi Joe,

just to be sure: which Scipion version are you using?

One of the ways to to this is to use scipion subset protocol with
intersection option:

1) first input will be your all dose-weighted mics (full set)
2) second input will be a set of mics (small one)

This will get you a dose-weighted subset of mics that you can use for
particle extraction (provide there either CTF subset or full CTF set, it
should work both ways).

Best regards,
Grigory

--------------------------------------------------------------------------------
Grigory Sharov, Ph.D.

MRC Laboratory of Molecular Biology,
Francis Crick Avenue,
Cambridge Biomedical Campus,
Cambridge CB2 0QH, UK.
tel. +44 (0) 1223 267542 <+44%201223%20267542>
e-mail: gs...@mr...

On Wed, Nov 29, 2017 at 8:58 PM, Joseph Lyons <ly...@mb...> wrote:

> Hi all,
>
>
> I have a data set comprised of dose-weighted and non-dose-weighted
> micrographs.  CTF estimation was performed on the non-dose-weighted group
> and I have selected a set of micrographs from this set based on CTF. ​
>
>
> The problem arises when I want to extract particles from the dose weighted
> set and assign the CTF from the smaller set to this process.
>
>
> When I use the smaller CTF set - it complains of missing CTF based on
> micName or micId. I was told that this would just allow particle extraction
> from the micrographs common to both the DW and CTF sets. Am I missing
> something?
>
>
> Best,
>
>
> Joe
>
>
>
>
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