[scipion-users] B-factor (over/undersharpening) - evaluation of the results

[Dmitry S. <sem...@gm...>]

Dear colleagues,

The question is about B-factor (over/under-sharpening)


How to evaluate the result of the postprocessing and find the optimum
B-factor?

In my case the B-factor was estimated automatically and equals ~ -112

I experimented with manual B-factor values ( -62; and -172) )and notices
that the process_guinier looks differently

Auto - case (B-factor= -112)

[image: Встроенное изображение 2]



B-factor= - 62


[image: Встроенное изображение 3]



B-factor= - 172

[image: Встроенное изображение 4]



So what seems to change more is AmplitudesSharpened - the red curve.

Should this curve be as flat as possible?
If there are the other parameters I have to look at?


Thank you!

Sincerely,
Dmitry