[scipion-users] movie alignments

[Dmitry S. <sem...@gm...>]

Dear colleagues,

The issue is again with movie correction scripts.

1. Motioncorr2
As input, I use the movie stacks (can I use corrected movies as an input to
increase the quality - for example after xmipp3-correlation alignment or it
will be repetition?) and get the error  -

ProgrammingError: Incorrect number of bindings supplied. The current
statement uses 32, and there are 22 supplied.
(the git was updated)

[image: Встроенное изображение 1]

How can I fix that?


2. xmipp3 - correlation alignment / optical alignment




2.1 correlation alignment

- from time to time the protocol stops. When I resubmit it continues
working.
But the question is why does that happen: problem with my dataset and/or
bug?


2.2 optical alignment

- if you may remember the problem was that it finished because of an error
in 1 file.

Is there already the solution for skipping the movies that causing the
error?

Alternatively, I removed that file (it looked fine in terms of frames and
visibility - I can send it for the analysis) and have to rerun the
xmipp3-correlation again. But there is no guarantee that the program will
not stop on some other file.



3. grigoriefflab - Input - movie stacks

stops in the beginning with error -

Input stack filename
14274:
[Runs/022622_ProtUnblur/tmp/movie_000010/FoilHole_26594251_Data_25564934_25564935_20160826_2014-21929.mrc]
: 24
14275:
14276:    No Such File - please enter a valid filename




[image: Встроенное изображение 2]



Sincerely,
Dmitry