Re: [scipion-users] scipion 1.1 & localrec 1.2.0 usage question

[Jose M. de la R. T. <del...@gm...>]

Hi Wolfgang,

The 'hasAlignmentProj' is a property that SetOfParticles can have, it means
that particles have projection-alignment information, i.e, the Euler angles
to make a reconstruction. This information is required in this protocol
because localrec will take that information together with the symmetry and
the vectors to generate another SetOfParticles (called sub-particles here)
with another angular information (also projection-alignment). Can you check
that the 'projection-alignment' is properly set after the import of
particles. If you select the import job, you can click in the 'db' icon and
the 'alignment' property under the outputParticles object. Have you
imported the star file of the particles after 3D refinement or
classification?

Hope this helps,
Jose Miguel.


On Mon, Apr 24, 2017 at 3:39 PM, Wolfgang Lugmayr <
wol...@im...> wrote:

> dear all,
>
> we can run localrec in the given configuration from the commandline (by
> adding some environment settings).
>
> when we try to use it from the gui we have the following problem:
> -> view: localized reconstruction
> -> 1. compute sub-particles
> -> input particles
>
> we cannot choose our imported particle file (from relion).
>
> what step must be done before that this particle set shows up?
> or whats means "SetOfParticles (condition: hasAlignmentProj)"?
>
> thanks in advance for any related comments,
> cheers,
> wolfgang
>
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