Re: [scipion-users] xmipp3-align with cl2d - saving subset error?

[Grigory S. <sha...@gm...>]

Hi Dmitry,

I believe this is related to the bug
<https://github.com/I2PC/scipion/issues/636> you reported last year.
Unfortunately, it is not fixed yet.

If you simply want to center your picked particles, I would suggest "spider
- ap sr" or "align pairwise" protocols, they are very quick but might
require a bit of parameter tuning. Both are described
<https://github.com/I2PC/scipion/wiki/Integrated-Protocols#spider-10-protocols>on
scipion wiki

Best regards,
Grigory

--------------------------------------------------------------------------------
Grigory Sharov, Ph.D.

MRC Laboratory of Molecular Biology,
Francis Crick Avenue,
Cambridge Biomedical Campus,
Cambridge CB2 0QH, UK.
tel. +44 (0) 1223 267542 <+44%201223%20267542>
e-mail: gs...@mr...

On Fri, Apr 7, 2017 at 11:15 AM, Dmitry Semchonok <sem...@gm...>
wrote:

> Dear colleagues,
>
> The question is about xmipp3-align with cl2d protocol
>
> I use this protocol in order to correct the picked particles - center them.
>
> After the protocol finished I tried to save the subset - namely all
> particles in the new set for the later processing in the Relion 2D. But
> that did not work. The new set creation ends up with error.
>
> Is it a problem in my data or a bug?
>
> [image: Встроенное изображение 1]
>
> [image: Встроенное изображение 2]
>
> Sincerely,
> Dmitry
>
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