Re: [scipion-users] exporting an EMX file to cryoSparc

[Jose M. de la R. T. <del...@gm...>]

Hi Juha,

Thanks for your feedback. Could you do a small experiment to detect if the
exported EMX file is correct? Could you import back the EMX file into
Scipion and try to reconstruct only with Relion (there is a protocol for
that) and check if it makes sense or not. Take into account that the map
probably will not be exactly the same that originally came out from Relion,
since it is filtered.

Anyway we will also check about this issue.

Bests,
Jose Miguel


On Fri, Mar 3, 2017 at 9:46 AM, Juha Huiskonen <ju...@st...> wrote:

> Hello
>
> I was wondering if the EMX export should work and if others have managed
> to use it with cryoSparc?
>
> I exported my dataset and imported EMX files to cryoSPARC after running
> Relion 3D refinement through Scipion. Import to CryoSparc seemed to work
> (it created a gallery of the images) but results from the first ab initio
> run were nonsensical.
>
> Next I tested importing MRC files and a corresponding STAR file (manually
> converting the same particle stacks to MRC and providing them together with
> a corresponding Relion STAR file from the refinement run). After the same
> run in cryoSparc, the results were as expected.
>
> Any ideas? Apologies if this is likely to be an issue on the cryoSPARC
> side, in this case I would  naturally contact those developers.
>
> Best wishes,
> Juha
>
>
>
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