[scipion-users] exporting an EMX file to cryoSparc

[Juha H. <ju...@st...>]

Hello

I was wondering if the EMX export should work and if others have managed to
use it with cryoSparc?

I exported my dataset and imported EMX files to cryoSPARC after running
Relion 3D refinement through Scipion. Import to CryoSparc seemed to work
(it created a gallery of the images) but results from the first ab initio
run were nonsensical.

Next I tested importing MRC files and a corresponding STAR file (manually
converting the same particle stacks to MRC and providing them together with
a corresponding Relion STAR file from the refinement run). After the same
run in cryoSparc, the results were as expected.

Any ideas? Apologies if this is likely to be an issue on the cryoSPARC
side, in this case I would  naturally contact those developers.

Best wishes,
Juha