Re: [scipion-users] 2 movie alignments - correlation and optical

[Carlos O. S. <co...@cn...>]

Dear Dmitry,


On 02/16/17 12:01, Dmitry Semchonok wrote:
> Dear colleagues,
>
> I would like to ask about 2 movie alignments - correlation and optical.
>
> When we do the movie alignment using these 2 protocols isn't there a 
> danger that the signal becomes weaker due to several interpolations?
The correlation protocol does not interpolate the images, it simply 
writes the shifts associated to each frame. Then, optical alignment 
compose these global shifts with the local shifts and perform a single 
interpolation at the very end, which is performed with cubic B-splines 
trying to achieve the highest possible quality.

Cheers, Carlos Oscar
>
>
> Sincerely,
> Dmitry
>
>
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Carlos Oscar Sánchez Sorzano                  e-mail:   co...@cn...
Biocomputing unit                             http://biocomp.cnb.csic.es
National Center of Biotechnology (CSIC)
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