[scipion-users] 1. binning; 2. cryoSPARC

[Dmitry S. <sem...@gm...>]

Dear colleagues,

could you please explain me the strategy of binning usage?

Apart of the fact that binning speeds up the processing. I would like to
use the binning to see my particles better (some of my data is very close
to focus)

In the SCIPION tutorial - Mix-and-Match Tutorial
<https://github.com/biocompwebs/scipion/wiki/tutorials/scipion_tutorial_betagal.pdf>
on the page 12 stands the *Preprocess particles protocol *that sugests to
do binning before CTF estimation.

My questions are:

1. When do you do unbinning of the dataset?

2. Do you also need to estimate the CTF for the unbinned dataset?

3. How / when do you do the binning if you work with movies (stucks) -
after the motion correction?




Another issue:

there is a new soft calling cryoSPARC

see the link about cryoSPARC
<http://www.nature.com/nmeth/journal/vaop/ncurrent/full/nmeth.4169.html?WT.feed_name=subjects_systems-biology>


Do you have plans to incorporate it in the SCIPION?


Sincerely,
Dmitry