Re: [scipion-users] auto-refinement for 2D

[Carlos O. S. <co...@cn...>]

Dear Dmitry,

as far as we understand, there is not such a thing as 2D 
auto-refinement. You may select a subset of classes and reclassify them 
using a different classifier (in this regard you may use Relion 2D to 
group bad particles in a few classes, and Xmipp CL2D to classify the 
good images, CL2D cores and stable cores also help to remove bad 
particles). Is that what you refer to?

Kind regards, Carlos Oscar


On 01/11/17 11:52, Dmitry Semchonok wrote:
>
> Dear colleagues,
>
>
> *? --> auto-refinement for 2D*
>
>
> We work a lot with negative staining samples doing classification (in 
> relion 2D) and getting 2D projection average classes.
>
>
> It might be possible to*improve 2D results* doing *auto-refinement* 
> step (for 2D).
>
>
> If yes how can we do that?
>
>
> I tried to use dataset and created 2D mrc (vol) file as a reference 
> but the program ended up with some errors.
>
> Dear colleagues do you have any ideas about that possibility ?
>
> Sincerely,
>
> Dmitry
>
>
>
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Carlos Oscar Sánchez Sorzano                  e-mail:   co...@cn...
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