[scipion-users] Fwd: Fwd: about RCT-Can I generate a tilted particle set according to an untilted class set?

[Carlos O. S. <co...@cn...>]

Dear Liz,

as you indicate, the RCT protocol is only capable of reconstructing 
volumes from individual untilted classes. To merge them, you may use any 
subtomogram averaging protocol. Currently in Scipion the only one fully 
available (there are some other in development) is cltomo (do CTRL-F to 
locate it). The number of classes would be 1 in your case.

Kind regards, Carlos Oscar


-------- Forwarded Message --------
Subject: 	[scipion-users] about RCT-Can I generate a tilted particle set 
according to an untilted class set?
Date: 	Wed, 11 Jan 2017 11:54:40 +0800 (CST)
From: 	ZhuLi <ga...@16...>
To: 	sci...@li...



Dear colleagues，

When I did RCT with scipion, I found that it could only generate volumes 
according to the classified untilted sets (like what you only need to 
import in the " Random Conical Tilt" menu are the tilt_pairs and 
Good_classes files ). Since after viewing those volumes, I need to merge 
a few classes to generate a better volume which may come from the same 
conformation. Then, what should I do to extract and merge those tilted 
particles from corresponding classes? (as you know, the classes were 
generated from untilted particles.)

Hope I described my question clearly.

Thanks.

Liz