Re: [scipion-users] protocol grigoriefflab - unblur

[Jose M. C. <ca...@cn...>]

Dear Dimitry,

Regarding local and non-local correction, like Optical Flow, in the context
of last year Map Challenge, we could show that Optical Flow always produced
equal or better results than non-local approach. At the time we did not use
dose compensation, which should improuve on top.

Josue Gomez has all the test data on our side, that were indeed compiled
but never published

Wbw..JM

On Wed, Nov 23, 2016 at 7:35 AM, Carlos Oscar Sorzano <co...@cn...>
wrote:

> Dear Dmitry,
>
> as far as I remember there is no serious comparison about different
> strategies of dose compensation, frame alignment (either at the level of
> micrograph as in cross correlation/optical flow or at the level of
> particle, polishing). Assuming that any combination is equivalent to any
> other combination is not reasonable, but unfortunately, I don't have an
> answer to which strategy is the best. Each one showed in their respective
> publications, that that action alone was improving resolution, but there
> has been nothing on the comparison or the combination of them.
>
> Kind regards, Carlos Oscar
>
> On 22/11/2016 11:55, Dmitry Semchonok wrote:
>
> Dear colleagues,
>
> 1 more question
>
> How do you use the *protocol grigoriefflab - unblur*?
>
> After the *movie alignment protocol*?
>
> How important is to use this protocol?
>
> Isn't the particle polishing will in a way do the same?
>
>
> Thank you
>
> Sincerely,
> Dmitry
>
>
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> Carlos Oscar Sánchez Sorzano                  e-mail:   co...@cn...
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-- 
Prof. Jose-Maria Carazo
Biocomputing Unit, Head, CNB-CSIC
Spanish National Center for Biotechnology
Darwin 3, Universidad Autonoma de Madrid
28049 Madrid, Spain


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