Re: [scipion-users] protocol grigoriefflab - unblur

[Carlos O. S. <co...@cn...>]

Dear Dmitry,

as far as I remember there is no serious comparison about different 
strategies of dose compensation, frame alignment (either at the level of 
micrograph as in cross correlation/optical flow or at the level of 
particle, polishing). Assuming that any combination is equivalent to any 
other combination is not reasonable, but unfortunately, I don't have an 
answer to which strategy is the best. Each one showed in their 
respective publications, that that action alone was improving 
resolution, but there has been nothing on the comparison or the 
combination of them.

Kind regards, Carlos Oscar


On 22/11/2016 11:55, Dmitry Semchonok wrote:
> Dear colleagues,
>
> 1 more question
>
> How do you use the *protocol grigoriefflab - unblur*?
>
> After the *movie alignment protocol*?
>
> How important is to use this protocol?
>
> Isn't the particle polishing will in a way do the same?
>
>
> Thank you
>
> Sincerely,
> Dmitry
>
>
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Carlos Oscar Sánchez Sorzano                  e-mail:   co...@cn...
Biocomputing unit                             http://biocomp.cnb.csic.es
National Center of Biotechnology (CSIC)
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