Re: [scipion-users] movie alignment

[Jose M. de la R. T. <del...@gm...>]

Dear Dmitry,

Defining which is "the best" is nearly impossible in most contexts. As you
may know, some algorithms perform better than other for some cases, but not
for all situations.
Following are some comments from our experience here in our lab, other may
have a totally different opinion.

We have extensively tested MotionCor and Optical Flow, which are both
integrated into Scipion. Optical Flow is quite a sophisticated approach to
handle local movements, and it was a joint work with Li and Yifan. .. More
about it at:

Alignment of direct detection device micrographs using a robust Optical
Flow approach. <http://www.ncbi.nlm.nih.gov/pubmed/25681631>
Abrishami V, Vargas J, Li X, Cheng Y, Marabini R, Sorzano CÓ, Carazo JM.
J Struct Biol. 2015 Mar;189(3):163-76.


Further along this line, during this year we have processed most of the Map
Challenge data sets with both methods, and the empirical conclusion that we
are reaching so far is that:


-For Falcon data: The combination of MotionCor and Optical Flow always
gives better results than MotionCor alone

-For K2 data: MotionCor works very well and Optical Flow just does very
little improvement.


Note that MotionCorr2 is already integrated into Scipion(in devel branch,
thanks to Grigory Sharov), but we have not extensively tested yet.


Hope this helps,

Jose Miguel.




On Sat, Sep 10, 2016 at 10:58 PM, Dmitry Semchonok <sem...@gm...>
wrote:

> Dear colleagues,
>
> What protocol by your experience is the best for the alignment of movies
> (if you use K2)?
>
> Sincerely,
>
> Dmitry
>
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