Re: [scipion-users] reference based particle picking

[Jose M. de la R. T. <del...@gm...>]

Dear Dmitry,

For doing what you want, there are 3 steps:

1) Create a set of projection in the command line.
Use 'scipion xmipp_angular_project_library', this Xmipp program mainly
requires the input Volume, the output stack and the distance in degrees
between projections.
Additionally, you can provide symmetry if known. (We could easily add a
protocol wrapping in the future)

2) Import the generated projections in 1) as SetOfAverages (protocol -
import averages)

3) Select any picking that use references (e.g., Relion autopicking(two
steps), IGBMC GemPicker or Gautomatch)

Hope this helps
Jose Miguel.


On Wed, Sep 7, 2016 at 11:42 AM, Dmitry Semchonok <sem...@gm...>
wrote:

> Dear colleagues,
>
> Could you please tell me how to create a (multi)reference for the particle
> picking?
>
> (I have 3d model of the required particle)
>
> Thank you!
>
> Sincerely,
> Dmitry
>
>
>
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