[scipion-users] Relion star files are wrong?

[Grigory S. <sha...@gm...>]

Hello!

I have noticed that in scipion-produced relion star files (after 2d
classification relion protocol) the_rlnMagnification column is wrong (it
prints nominal 59000 instead of actual 31818) and there is no
_rlnDetectorPixelSize column in output star files.

That's why I wonder what values should be used for Magnification during
Micrograph import protocol?

I have used 59000x and 1.1A/px ("from image") during import. Classification
was done on C4 data, hence pixel size 4.4A. Actual mag should be then
4.4/14um=31818 and not 59000 in star file, given our detector size 14um.

Best regards,
Grigory

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Grigory Sharov, Ph.D.
Institute of Genetics and Molecular and Cellular Biology
Integrated Structural Biology Department (CBI)
1, rue Laurent Fries
67404 Illkirch, France
tel. 03 69 48 51 00
e-mail: sh...@ig...