'No read belongs to the gene family! No contig is generated!

Scalable and accurate targeted gene assembly for large-scale NGS data

Status: Alpha

Brought to you by: zhangy72

'No read belongs to the gene family! No contig is generated!

Forum: General Discussion

Creator: Dave Roe

Created: 2014-08-24

Updated: 2014-08-26

Dave Roe - 2014-08-24

I get the following message when I run via:
SAT-Assembler.sh -m test.hmm -f test.fasta -o test
I am confident that the HMM was build with reference sequences that are close to the fasta sequences. Any suggestions on how I should debug my problem?

Traceback (most recent call last):
File "/Users/daver/bin/SAT-Assembler/assembler.py", line 2203, in <module>
main()
File "/Users/daver/bin/SAT-Assembler/assembler.py", line 2158, in main
sys.stderr('No read belongs to the gene family! No contig is generated!')
TypeError: 'file' object is not callable

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Yuan Zhang - 2014-08-26

Is the HMM built from protein sequences to which the NGS data belong?

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