Home

Introduction

• SWATH-Auto System Analyzer Tool, SASA Tool , is a novel SWATH platform for non-targeted metabolomics data analysis with accurate mass spectral library for metabolite identification using SWATH acquisition mode. Data analysis is based on scoring the alignment probability of certain precursor with its possible transition signals. This strategy is operated via unbiased SWATH based approach, in cyclic recording, over the acquisition time range of sequential survey scans and precursors transition ion spectra in predetermined isolation windows. The validated platform utilizes mass spectral library of high-resolution small molecules parsed from the HMDB for both positive and negative modes. The platform is well tested and validated manually using authentic standards and exclusion list. The fidelity of the workflow and the applicability of the library were tested using various biological samples; plasma, urine, and cell lines.

• The software process workflow consists of 4 main steps; 1) Database choice step; where the user has to select and/or generate a database. 2) Parents detection step using MasterView; SASA speeds up the search through generation MasterView method, 3) Fragments detection step using MultiQuant; SASA will calculated the start and end masses, experimental window, and generate MultiQuant method , 4) Parents and fragments filtration; final step where the filtration process is applied using accepted predetermined parameters.

• The whole workflow are documented in this video tutorial: SASA video tutorial