Re: [Pytom-mail] Run localization at specific points

[Irene De t. <ire...@em...>]

Hi Dustin,

Thanks a lot for your answer! 

I was looking for a direct solution, since I am interested in doing template matching at around 2000 points, rather than the  ~10^8 voxels that the full tomogram has...

Is there some script available to do something like this?

Thanks!
Irene

> On Jul 11, 2018, at 4:47 PM, Dustin Morado <dus...@gm...> wrote:
> 
> Hi Irene,
> 
> The best way I know of how to do this is to mask the output scores volume before picking peaks.
> You can make a mask by binary thresholding etc. We have done this to discard the false peaks we often
> get at the air-water interface by masking out the edges of the score volume before peak picking.
> 
> HTH
> ___
> 
> Cheers,
> Dustin M.
> 
> 
>> On Wed, Jul 11, 2018 at 3:35 PM Irene De teresa <ire...@em...> wrote:
>> Hello all,
>> 
>> I am new in PyTom and I am interested in running a template matching algorithm, but only at very specific points in a tomogram.
>> 
>> I was checking how to modify the localization job that is provided by the PyTom tutorial (pytom.org/doc/pytom/tutorial.html), but I couldn’t manage to indicate the list of points at the tomogram where I want to compute the cross-correlation coefficients with respect to the reference particle. Does anyone have an idea of how could I do it?
>> 
>> Thanks a lot in advance!
>> Best,
>> 
>> Irene
>> 
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