[Pytom-mail] (no subject)

[<da...@st...>]

Hi all, 

We've recently been playing with PyTom a bit more to do some subtomogram averaging, and a couple of questions have come up: 

1)  When using the WienerFilterAlignment.py, what precisely is required for the CTFSquared parameter? We're not entirely sure what it is asking for, or how to go about making that volume. Is it akin to a missing wedge mask made with ctf slices, like in relion subtomo? 

2) When using GFRMAlignment.py, how do you give symmetry parameters? Digging through the code, I think there should a <MultiSymmetries> tag in the job xml file, but I'm not sure how to fill this out correctly. 

Any help would be great!

Cheers,
Daven