Re: [Pytom-mail] FRMAlignment - New

[Yuxiang C. <che...@gm...>]

Hi Dustin,

that means for some reason the determined resolution (according to the set
FSC value) is too high, which cannot be true.
Most likely the particles were aligned to the missing wedges. Please check
the wedge settings in your particles.
If the wedges are set correctly and the problem still occurs, I'd suggest
to set the wedges to empty to avoid aligning towards missing wedge
direction.

Cheers,
Yuxiang



On Sat, Feb 20, 2016 at 2:47 AM, Dustin Morado <dus...@gm...>
wrote:

> Hi all,
>
> I keep getting the following termination to my FRM jobs
>
> current resolution 40.4014545455 1.03125
> New frequency too high. Terminate!
>
> My Job field has the following:
> <FRMJob PeakOffset="4" BandwidthRange="[4, 32]" Frequency="6"
> Destination="." MaxIterations="10" RScore="False" WeightedAverage="False"
> BFactor="None" AdaptiveResolution="0.1" FSC="0.5”>
>
> My particles are 64x64x64 with a 2nm pixel size so for some reason even if
> I set the initial frequency to an incredibly low resolution I still get
> this same abort. I have tried changing the frequency and the bw_range, but
> the only way I can get it to run for more than one iteration is to set the
> FSC to a very high value ~0.75+.
>
> Any suggestions?
>
> —
> Cheers,
> Dustin
>
>
>