[PyMOL] Nonsense mutagenesis

[Jevin P. <jev...@pe...>]

Hi there,

I’m trying to conduct some mutagenesis on a protein, but I have found that PyMol only currently does mutagenesis from one amino acid to another. Is there a way to conduct mutagenesis for point mutations resulting in a termination codon (eg. p.Gln415Ter)?

Addtionally, I am unsure how to do nucleotide mutagenesis on a PDB file that only had an AA sequence. Is there a way to change the amino acid sequence to a nucleotide sequence?

Thank you in advance!

Jevin Parmar