Re: [PyMOL] [EXTERNAL] basic trouble viewing electron density maps

[criss.hartzell <cri...@gm...>]

That explains it! Thanks. I guess I need a tutorial on how to look at EM maps. 

Criss Hartzell


> On Oct 27, 2021, at 12:26 PM, Brown, Zuben <zb...@cu...> wrote:
> 
> 
> They are different types of maps.
> 
> 2F67 -- X-ray map
> 7B5C -- EM map
> 
> 7B5C is not a 2fofc map.
> 
> Best wishes,
> Zuben
> From: Criss Hartzell <cri...@gm...>
> Sent: Wednesday, October 27, 2021 2:51 PM
> To: Pymol User list <pym...@li...>
> Subject: [EXTERNAL] [PyMOL] basic trouble viewing electron density maps
>  
> I am having trouble viewing electron density maps. 
> Everything works fine if I use the protein 2F67. But with 7B5C, I am stumped.
> 
> fetch 7b5c, type=2fofc gives me an error: 
> Warning: failed to fetch from https://www.ebi.ac.uk/pdbe/coordinates/files/7b5c.ccp4
> 
>  Error-fetch: unable to load '7b5c'.
> If I go to EMDB and download the map file (EMD_12025) and load it manually:
> (set normalize_ccp4_maps, 0
> load H:/.../emd_12025.map, \emd_12025
> isomesh emd_12025_isomesh, emd_12025, 1.0)
> The screen is blank and changing levels, etc. does nothing.
> But it works in UCSF Chimera. What am I missing?
> Thanks.
> Criss
> 
>