Re: [PyMOL] CCP4 maps not aligning to my PDB file

[Tamas H. <bio...@gm...>]

Hi,

PyMOL seems not to primarily target working with densities.

I suggest to use Chimera if you work with cryo EM maps.
E.g. you can use it to fit your struct into the density map.
If you decide to use Chimera, be careful, a first step of frustration 
could be caused by the extension of the density file. Yes, the extension 
- .mrc or .map; depending on the extension the map is aligned to the 
origo or not (or something like that).

Bests,
Tamas

On 4/12/21 8:34 AM, shubhashish chakraborty wrote:
> Hello,
> I am not able to align my pdb file with its respective electron 
> density map generated by CCP4i. Kindly let me know what is going wrong.
> An image is attached for better reference.
>
> Thank you
> Shubhashish Chakraborty
> PhD Research Scholar (SRF)
> Varma Lab
> Structural and Molecular biology Lab
> Advanced Centre for Treatment, Research and Education in Cancer (ACTREC)
> Khargar, Navi Mumbai
>
>
>
>
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