Re: [PyMOL] mutations

[Noinaj <no...@uk...>]

Orla,

This is simply, for the most part.  Here is what I do. =20

Load your *.pdb file.  Then under WIZARD in the menu bar, select =
MUTAGENESIS.  Then pretty much, follow directions.  In the PyMOL VIEWER =
window, it will ask you to ' pick a residue.'  Once you pick a residue, =
select the type of residue you want to mutate to by left clicking on [No =
Mutation] button and holding down (under Mutagenesis).  This brings up a =
list of residues that you can then select from.  Once you select the =
residue you want, hit APPLY, then DONE.  If everything was done =
correctly, you should see your mutated residue. =20

There are probably other ways to do this, but I hope this helps.  If you =
would like screenshots of the steps, just let me know. =20



Cheers,
Nick


  ----- Original Message -----=20
  From: Orla O'Sullivan=20
  To: pym...@li...=20
  Sent: Tuesday, April 18, 2006 11:31 AM
  Subject: [PyMOL] mutations


  Hi all

  I'm a newbie to Pymol and so far have been very impressed. However I =
have tried to mutate some residues in my structure and have hit a brick =
wall.

  Has anyone done this with pymol and let me know how to do it?

  =20

  Regards=20

  =20

  Orla=20

  =20

  Dr.Orla O'Sullivan

  Research Officer

  Biotechnology

  =20

  =20

  Moorepark Food Research Centre
  Teagasc

  Moorepark
  Fermoy, Co. Cork
  Ireland

  =20

  Tel:       +353 - (0)25 - 42344

  =20