#380 CLASH

[simona panni]

It is a method to identify RNA-RNA interactions that occur in the proximity of an RNA binding protein of interest. The RNA is crosslinked to the protein, that is immunoprecipitated. After RNAsi treatment the RNA hybrids protected from degradation by the protein are ligated and chimeras are sequenced.

Reference
Grzegorz Kudla1,2, Sander Granneman1, Daniela Hahn1, Jean D. Beggs, and David Tollervey.
Cross-linking, ligation, and sequencing of hybrids reveals RNA–RNA interactions in yeast
Proc Natl Acad Sci U S A. 2011 Jun 14;108(24):10010-5. PMID: 21610164

[Pablo Porras]

This issue handled with the batch of terms created in issue #379.