From: Ratna R T. <rat...@es...> - 2013-10-25 14:43:34
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Hi, I converted the Thermo LTQ Orbitrap Velos RAW files MGF using NIST - ReAdw4Mascot (a modified version of ReAdW). Then I searched a human fasta database using latest MS-GF+ successfully. I am planning to run peptideprophet and proteinprophet to validate the assignments and group proteins in trans-proteomic pipeline (TPP). When I tried to convert the resulting mzid files to pepXML using idconvert for use in TPP, I am getting the following error. ========== idconvert TCGA-A6-3807-01A-22_W_VU_20121019_A0218_4I_R_FR01.raw.IT.cid.ch.mzid --pepXML format: pepXML outputPath: . extension: .pepXML filenames: TCGA-A6-3807-01A-22_W_VU_20121019_A0218_4I_R_FR01.raw.IT.cid.ch.mzid processing file: TCGA-A6-3807-01A-22_W_VU_20121019_A0218_4I_R_FR01.raw.IT.cid.ch.mzid writing output file: ./TCGA-A6-3807-01A-22_W_VU_20121019_A0218_4I_R_FR01.raw.IT.cid.ch.pepXML Error writing analysis 1 in TCGA-A6-3807-01A-22_W_VU_20121019_A0218_4I_R_FR01.raw.IT.cid.ch.mzid: [identdata::digestedPeptide] invalid pre/post on PeptideEvidence element ============ Can you take look. I appreciate any inputs. Thanks in advance Rajesh |