#22 Probes (internal oligos) can be designed in excluded regions

Solved
closed
nobody
5
2016-03-06
2007-12-07
richard
No

Internal oligos in Primer3 don't respect the excluded region option. To reproduce, just run a design with "pick hybridization probe" then go back and add an excluded region to somewhere in the binding region of the probe and run design again.

Restult:

1 TAGGGTTGTAAAACTCTTTCGCCGGTTAGGATAATGACGTTAACCGGAGAAGAAGCCCCG
>>>>>>>>>>>>>>>>>>>>>>>> ^^^^^XXXXXXXXXX^^^^^

61 GCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGGGCTAGCGT
<<<<<<<<<<<<<<<<<<<

Discussion

  • Untergasser

    Untergasser - 2016-03-06
    • status: open --> closed
    • discussion: enabled --> disabled
    • Group: --> Solved
     

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