From: SourceForge.net <no...@so...> - 2012-12-02 15:37:01
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Chado item #3590723, was opened at 2012-11-28 08:01 Message generated for change (Comment added) made by gomidori You can respond by visiting: https://sourceforge.net/tracker/?func=detail&atid=2096276&aid=3590723&group_id=65526 Please note that this message will contain a full copy of the comment thread, including the initial issue submission, for this request, not just the latest update. Category: None Group: None Status: Open Resolution: None Priority: 7 Private: No Submitted By: Midori Harris (gomidori) Assigned to: Kim Rutherford (kim_rutherford) Summary: 'from=' data missing from GAF for IC Initial Comment: Problem! The GAFs being written out from Chado don't have anything in column 8 (with/from) where the evidence (col 7) is IC. I used pombase-build-2012-11-25-v2-l1.gaf to the GAF in the GO repository. I looked a couple other GAFs from the dumps as well, and none had the data (tried pombase-build-2012-10-19-v1-l1.gaf, pombase-build-2012-11-05-v1-l1.gaf, pombase-build-2012-11-18-v2-l1.gaf). I didn't try to chase up every annotation, but the handful I spot-checked all had the requisite data in the embl files. Just one example: FT /GO="aspect=P; term=histone acetylation; GOid=GO:0016573; FT evidence=IC; db_xref=GO_REF:0000001; from=GO:0035267; FT date=20090301" becomes PomBase SPAC1F5.11c tra2 GO:0016573 GO_REF:0000001 IC P NuA4 complex phosphatidylinositol pseudokinase complex subunit Tra2 protein taxon:4896 20090301 PomBase The GO filter-gene-association.pl script is totally supposed to catch these, but equally totally failed -- it told me "Congratulations, there are no errors." and I didn't realize until today that it was lying. I'm reporting that to GO. ---------------------------------------------------------------------- >Comment By: Midori Harris (gomidori) Date: 2012-12-02 07:36 Message: That looks MUCH better - thanks! I'll fix the problem lines, and sooner or later GO SVN will be back among the living and I'll commit the new GAF ... ---------------------------------------------------------------------- Comment By: Kim Rutherford (kim_rutherford) Date: 2012-12-02 00:21 Message: This is fixed in the latest load: http://curation.pombase.org/kmr44/dumps/pombase-build-2012-11-28-v3-l1/ Sorry, the GAF writer was ignoring the froms. The fix added the from to most of the IC annotations. I've made a file with the ones that look like problems: Dropbox/pombase/Chado/GAF_IC_annotation_problems.txt There's only a handful. Some have no from, some have a from that isn't a GO term (maybe that's OK?) and there is one IMP annotation that has a from in Chado, but perhaps shouldn't. ---------------------------------------------------------------------- You can respond by visiting: https://sourceforge.net/tracker/?func=detail&atid=2096276&aid=3590723&group_id=65526 |