From: SourceForge.net <no...@so...> - 2011-04-04 16:18:56
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Curation tasks item #3274373, was opened at 2011-04-04 16:18 Message generated for change (Tracker Item Submitted) made by val_wood You can respond by visiting: https://sourceforge.net/tracker/?func=detail&atid=2096421&aid=3274373&group_id=65526 Please note that this message will contain a full copy of the comment thread, including the initial issue submission, for this request, not just the latest update. Category: None Group: None Status: Open Resolution: None Priority: 5 Private: No Submitted By: Valerie Wood (val_wood) Assigned to: Nobody/Anonymous (nobody) Summary: mating type region update Initial Comment: Need to follow this up at some point. MAy need to check further with Juan... Yes, what Xavi did is perfect. He's put together mat2 and mat3 with adjacent regions (which should be present in all h90 strains). In h90 cells, mat1 would switch between having a p cassette or a m cassette. In 972 h- there is a deletion of mat2-p. However, in some h+ strains there is a deletion of mat3-M, but more frequently there are mutations that prevent the switching from P to M, although mat2-P is still there. Therefore, one would have to assemble a different region for each strain. I think the configuration of the different h+ and h- strains is described in the very old pombe book. Anyway, most of the time people don't even know which h+ or h- allele they have. I think it would help to annotate mat1-m and mat3-m as such in the main contig (rather than calling them matmi_1 and _2 - or at least add a note), so that people know which one they are looking at. Also, it would help to annotate the features of the mating type region that Xavi put in his contig and that are present in 972 (like the IR-L and IR-R regions, or the homology boxes). Finally, would it be possible to add a note somewhere directing people to the mating type contig? I hope this is useful - let me know if I can help with anything else Juan ---------------------------------------------------------------------- You can respond by visiting: https://sourceforge.net/tracker/?func=detail&atid=2096421&aid=3274373&group_id=65526 |