#18 Position selection

[lewis collins]

I really like the program, it's very useful and gives lots of options when designing primers. However, there is one thing I thought would be useful, certainly for me of late. Sometimes, it is necessary to design primers to specific regions and positions. I'm currently using primers to confirm RNAi knockdown in Drosophila and to assess inadvertent knockdown of other genes. I was wondering if it would be possible to create an option that would allow to select more specifically, e.g. to design the 5' primer between position 100-150 and the 3' primer somewhere between 250-300.

When designing these primers, it is important that the forward and reverse flank the area of interest and try to maintain a reasonable product length, certainly <1000bp