Is there anyway to show the program intron exon boundaries if the sequence wasn't downloaded from ensemble? I have a gene that is found in NCBI but not ensemble. By just putting the genomic seq on the left and mrna on the right, the program does not recognize boundaries. Any help? Thanks
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PerlPrimer should recognise the intron/exon boundaries if you do as you described. Are there any error messages displayed (please check the minimised console window as well)? If not, then the only explanation is that there aren't any introns in the sequence - are you sure you didn't paste the wrong sequence into the genomic box?
Cheers,
Owen
If you would like to refer to this comment somewhere else in this project, copy and paste the following link:
Is there anyway to show the program intron exon boundaries if the sequence wasn't downloaded from ensemble? I have a gene that is found in NCBI but not ensemble. By just putting the genomic seq on the left and mrna on the right, the program does not recognize boundaries. Any help? Thanks
Hi,
PerlPrimer should recognise the intron/exon boundaries if you do as you described. Are there any error messages displayed (please check the minimised console window as well)? If not, then the only explanation is that there aren't any introns in the sequence - are you sure you didn't paste the wrong sequence into the genomic box?
Cheers,
Owen