In the latest version, 1.1.21, things worked when first installing. However now when trying to retrieve a gene from ensemble, the gene can be searched and a transcript selected but when hitting OK to load the information into the perlprimer browser, I get two pop errors in succession (Error:500 Internal Server Error), followed by a third pop error message (Error: Please enter an mRNA sequence). The the status at the bottom of the perlprimer browser indicates that the gene had been retrieved successfully although no sequence information appears in genomic sequence or mRNA sequence windows of the browser. This occurs on all computers i've tried on both my work and home networks. All machines run windows XP.
Here is the message in the script window:
PerlPrimer v1.1.21
Copyright ⌐ 2003-2011 Owen Marshall
Designs primers for PCR, Bisulphite PCR, QPCR (Realtime), and Sequencing
This window is required for PerlPrimer to run -
you may minimise it but please do not close it
Please wait while PerlPrimer loads ...
PerlPrimer started successfully
destroy (1):
0 0x17b6a0c PV f=00080504 {}(3)(3)
SV = PV(0x22cf3b4) at 0x17b6a0c
REFCNT = 3
FLAGS = (PADBUSY,PADMY,ROK)
RV = 0x2320fe8
PV = 0x2320fe8 ""
CUR = 0
LEN = 0
Tk::Error: Usage $widget->destroy(...) at script/perlprimer.pl line 4959.
Tk callback for .toplevel3.frame1.frame.button
<ButtonRelease-1>
(command bound to event)
Have the same problem, using 1.1.21 on XP. Tried different species, same result
I have exactly the same problem as tdb0006. When I try to get a sequence from ensemble, a message of \"internal server error" popsup. I\'ve tried in 4 different computers and nothing works!.