#28 Trouble in bacteria ORF

[Marcelo]

Hi,

When I try to find a qPCR primers for a bacterium ORF, PerlPrimer doesn't find any one!

I think that the reason for that is that PerlPrimer recognize only ATG start codon. I'm not sure here!

Could you help me on the way to get PerlPrimer work with bacterial ORFs?

Thank you very much!

Here is an ORF example:

TAAGTGAAGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGCAGGCCTA
ACACATGCAAGTCGAACGGCAGCACAGTAAGAGCTTGCTCTTATGGGTGG
CGAGTGGCGGACGGGTGAGGAATACATCGGAATCTACTCTTTCGTGGGGG
ATAACGTAGGGAAACTTACGCTAATACCGCATACGACCTACGGGTGAAAG
CGGAGGACCTTCGGGCTTCGCGCGGTTGAATGAGCCGATGTCGGATTAGC
TAGTTGGCGGGGTAAAGGCCCACCAAGGCGACGATCCGTAGCTGGTCTGA
GAGGATGATCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGG
AGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCAT
GCCGCGTGGGTGAAGAAGGCCTTCGGGTTGTAAAGCCCTTTTGTTGGGAA
AGAAAAGCAGTCGGTTAATACCCGATTGTTCTGACGGTACCCAAAGAATA
AGCACCGGCTAACTTCGTGCCAGCAGCCGCGGTAATACGAAGGGTGCAAG
CGTTACTCGGAATTACTGGGCGTAAAGCGTGCGTAGGTGGTGGTTTAAGT
CTGTTGTGAAAGCCCTGGGCTCAACCTGGGAATTGCAGTGGATACTGGGT
CACTAGAGTGTGGTAGAGGGTAGCGGAATTCCCGGTGTAGCAGTGAAATG
CGTAGAGATCGGGAGGAACATCCGTGGCGAAGGCGGCTACCTGGACCAAC
ACTGACACTGAGGCACGAAAGCGTGGGGAGCAAACAGGATTAGATACCCT
GGTAGTCCACGCCCTAAACGATGCGAACTGGATGTTGGGTGCAATTTGGC
ACGCAGTATCGAAGCTAACGCGTTAAGTTCGCCGCCTGGGGAGTACGGTC
GCAAGACTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAG
TATGTGGTTTAATTCGATGCAACGCGAAGAACCTTACCTGGTCTTGACAT
CCACGGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACCGTGAGACAG
GTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCC
CGCAACGAGCGCAACCCTTGTCCTTAGTTGCCAGCACGTAATGGTGGGAA
CTCTAAGGAGACCGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCA
AGTCATCATGGCCCTTACGACCAGGGCTACACACGTACTACAATGGTAGG
GACAGAGGGCTGCAAACCCGCGAGGGCAAGCCAATCCCAGAAACCCTATC
TCAGTCCGGATTGGAGTCTGCAACTCGACTCCATGAAGTCGGAATCGCTA
GTAATCGCAGATCAGCATTGCTGCGGTGAATACGTTCCCGGGCCTTGTAC
ACACCGCCCGTCACACCATGGGAGTTTGTTGCACCAGAAGCAGGTAGCTT
AACCTTCGGGAGGGCGCTTGCCACGGTGTGGCCGATGACTGGGGTGAAGT
CGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT