#813 NTR - Bru-seq & BruChase-seq

[Jason Hilton]

editor preferred term: bromouride labeling and sequencing
alternate terms: Bru-seq
textual definition: RNAs recently labeled using bromouridine (Bru) are sequenced to reveal spans of nascent transcription in the genome.
definition source for the textual definition: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4009065/
logical definition: RNA-seq assay --> Bru-seq

editor preferred term: bromouridine pulse-chase and sequencing
alternate terms: BruChase-seq
textual definition: Following bromouridine (Bru) labelling, cells are chased in uridine for different periods of time, allowing for the isolation of RNA populations of specific ages for sequencing.
definition source for the textual definition: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4009065/
logical definition: RNA-seq assay --> BruChase-seq

[Chris Stoeckert]

Jason, how about these definitions to standardize them and emphasize the difference in objectives?
Bru-seq: An RNA-seq assay to identify spans of nascent transcription in the genome through isolation of recent bromouridine (Bru) labelled RNAs.
BruChase-seq: An RNA-seq assay to identify RNA populations of specific ages through isolation of RNAs first labelled with bromouridine (Bru) followed by chasing in uridine for different periods of time.
Thanks, Chris

[Jason Hilton]

Perfect.

[Chris Stoeckert]

terms added to OBI