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#790 New Term Request - RACE
editor preferred term: 5' rapid amplification of cDNA ends
alternate terms: 5' RACE
textual definition: The use of reverse transcription and PCR to amplify mRNA sequence between a defined internal site and the 5' end of the mRNA.
definition source for the textual definition: https://www.thermofisher.com/us/en/home/references/protocols/nucleic-acid-amplification-and-expression-profiling/cdna-protocol/5-race-system-for-rapid-amplification-of-cdna-ends.html
logical definition: enzymatic amplification --> 5' RACE
editor preferred term: 3' rapid amplifciation of cDNA ends
textual definition: The use of reverse transcription and PCR to amplify mRNA sequence between a defined internal site and the 3' end of the mRNA.
definition source for the textual definition: https://www.thermofisher.com/us/en/home/references/protocols/nucleic-acid-amplification-and-expression-profiling/cdna-protocol/5-race-system-for-rapid-amplification-of-cdna-ends.html
definition source for the textual definition:
logical definition: enzymatic amplification --> 3' RACE
editor preferred term: 5' RNA ligase mediated rapid amplification of cDNA ends
alternate terms: 5' RLM RACE
textual definition: A 5' RACE procedure that uses RNA ligase to add an RNA Adapter oligonucleotide to intact 5' mRNA ends, allowing the amplification of cDNA only from full-length, capped mRNA.
definition source for the textual definition: https://tools.thermofisher.com/content/sfs/manuals/cms_056070.pdf
logical definition: enzymatic amplifciation --> 5' RACE --> 5' RLM RACE
Jason, thanks for submitting these terms. We like to keep track of the projects that are contributing to and using OBI. Can you provide us some background?
Thanks, Chris
These are for project ENCODE. At the DCC, we recieved requests for these new terms at some time before I came on-board, so unfortunately, I cannot give specifics on these terms.
Are these meant to be a type of RT-PCR (i.e., an enzymatic amplification) or are they meant to be types of assays?
If an assay, what is the objective? transcription profiling/ transcript analysis?
They are meant to be types of RT-PCR.
Thanks. Will move ahead and put these in as such.
Would it also be appropriate to create a term for RACE, as follows?
editor preferred term: rapid amplification of cDNA ends
alternate terms: RACE
textual definition: The use of reverse transcription and PCR to amplify mRNA sequence between a defined internal site and the 5' or 3' end of the mRNA.
definition source for the textual definition: http://www.clontech.com/US/Products/cDNA_Synthesis_and_Library_Construction/RACE_Rapid_Amplification_of_cDNA_Ends/5_Prime_RACE_and_3_Prime_RACE
logical definition: enzymatic amplification --> RACE --> 5' RACE, enzymatic amplification --> RACE --> 3' RACE
discussed on Jan 11, 2016 obi call. Agreed to add under RT-PCR. WIll clean up textual definition to match standard form.