Welcome to the Nest-o-Patch program forum. I will be glad to see any ideas and suggestions about the development of the program or to answer your questions.
Last edit: Viatcheslav Nesterov 2013-09-12
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I've been playing around with Next-o-patch and I couldn't find a way to measure peak amplitudes in Whole cell. Is there any way for doing it? BTW, is it also pasible to fit the inactivation kineticks to a single or double exponentials?
Thanks
Paco
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There are two techniques to measure amplitudes in whole cell mode, both are described in the User Manual, chapter 3 section 5, p.42.
Here is the description:
There are several ways to measure Y-distance. The first one: set Borders. Their coordinates and
range will be shown in the Statusbar. Double-clicking on
the panel of Statusbar sends the data to the Notebook.
Another possibility is to set levels. In the Section 3.4.1 we mentioned setting of
levels from the Amplitude Tab; however, it is possible to set them directly in the
Trace Tab (see Figure 3.16, B).
Levels coordinates are automatically sent to the Notebook.
As for the fitting of kinetics inactivation, this is not yet possible. I plan to include it in one of the following versions of Nest-o-Patch.
Best regards!
Viatcheslav
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I'm having trouble loading macroscopic currents from whole cell recordings (collected with HEKA). After I load my file (either as .pul or .dat) I see '2' in the traces count window of the pulse tree tab. When I load the sweeps with trace count 1 it looks like it corresponds to the leak (I use leak subtraction during the recording), and when I load the sweeps with trace count set to 2 it corresponds to the voltage protocol. Am I doing something wrong? Sorry for the silly question and thanks in advance for any help you can provide!
-Travis
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Hi Travis!
Sounds like a bug. Could you please send me problematic file(s). Use "Contact the author" button of the program. I will try to find a solution.
Best regards,
Viatcheslav
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Welcome to the Nest-o-Patch program forum. I will be glad to see any ideas and suggestions about the development of the program or to answer your questions.
Last edit: Viatcheslav Nesterov 2013-09-12
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Hi Viatcheslav,
I've been playing around with Next-o-patch and I couldn't find a way to measure peak amplitudes in Whole cell. Is there any way for doing it? BTW, is it also pasible to fit the inactivation kineticks to a single or double exponentials?
Thanks
Paco
Hi Paco!
Thanks to the interest to the program!
There are two techniques to measure amplitudes in whole cell mode, both are described in the User Manual, chapter 3 section 5, p.42.
Here is the description:
There are several ways to measure Y-distance. The first one: set Borders. Their coordinates and
range will be shown in the Statusbar. Double-clicking on
the panel of Statusbar sends the data to the Notebook.
Another possibility is to set levels. In the Section 3.4.1 we mentioned setting of
levels from the Amplitude Tab; however, it is possible to set them directly in the
Trace Tab (see Figure 3.16, B).
Levels coordinates are automatically sent to the Notebook.
As for the fitting of kinetics inactivation, this is not yet possible. I plan to include it in one of the following versions of Nest-o-Patch.
Best regards!
Viatcheslav
View and moderate all "Features request and help" comments posted by this user
Mark all as spam, and block user from posting to "Discussion"
Hi Viatcheslav,
I'm having trouble loading macroscopic currents from whole cell recordings (collected with HEKA). After I load my file (either as .pul or .dat) I see '2' in the traces count window of the pulse tree tab. When I load the sweeps with trace count 1 it looks like it corresponds to the leak (I use leak subtraction during the recording), and when I load the sweeps with trace count set to 2 it corresponds to the voltage protocol. Am I doing something wrong? Sorry for the silly question and thanks in advance for any help you can provide!
-Travis
Hi Travis!
Sounds like a bug. Could you please send me problematic file(s). Use "Contact the author" button of the program. I will try to find a solution.
Best regards,
Viatcheslav
Bug fixed in the version 2.0.3.
Issue is closed.
Travis, once more thanks for reporting the bug!