NanoTrackJ Wiki
NanoTrackJ - Size characterization of freely diffusing nanoparticles
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For the purpose of testing, you can download some sample video files.
Video recording of freely diffusing 100nm polystyrene nanoparticles using nanoparticle tracking analysis
Video not available
NanoTrackJ settings which works well for this video:
Parameter | Value |
---|---|
Center estimator | Maxima |
Diffusion coefficient estimator | Covariance |
Min. expected particle size | 90 nm |
Search radius | 13.34 px |
Min. number of steps per track | 20 |
Temperature | 22,5 °C |
Pixel size | 164 nm |
Frame rate | 30 FPS |
Linear drift corrected | True |
Walker's method used | True |
Walker's method min size | 800 nm |
Mean size (Maxima Dialog) | 3 |
Tolerance (Maxima Dialog) | 15 |
Video recording of freely diffusing 60 nm and 80 nm gold nanoparticles using dark field microscopy
Video not available
NanoTrackJ settings which works well for this video:
Parameter | Value |
---|---|
Center estimator | Maxima |
Diffusion coefficient estimator | Covariance |
Min. expected particle size | 50 nm |
Search radius | 18.03 px |
Min. number of steps per track | 20 |
Temperature | 24 °C |
Pixel size | 182 nm |
Frame rate | 25 FPS |
Linear drift corrected | True |
Walker's method used | True |
Walker's method min size | 800 nm |
Mean size (Maxima Dialog) | 4 |
Tolerance (Maxima Dialog) | 12 |