From: <r00...@nt...> - 2019-12-12 14:32:57
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Dear mummer group: Good evening. Japanese apricot (Prunus mume) is our research specie, and we conducted 10x genomics technique making a new genome by using genomic DNA from Japanese cultivar. Furthermore, we would like to compare the genomic difference between publised Prunus mume genome (chinese cultivar) with the new 10xgenomics Prunus mume genome. But the result of mummerplot showed very strange from the demo picture from the mummer tutorial website. The steps we used as below: nucmer -p 191212mummer-10xNanko_oldPmref /data/share/genome/pmum/old-with/annotation-until2018/P.mume.genome.fa /data/public/hsiang/10x_sequencing/190812-test1-result/output_0817_prefix.1.fasta mummerplot 191212mummer-10xNanko_oldPmref.delta --postscript And the output files as below: out.ps out.rplot out.gp out.fplot 191212mummer-10xNanko_oldPmref.delta FILES LINK: https://drive.google.com/open?id=1wH1Xpt8r4MiZ050UPWWu0mUkeJFpZEqH Could you give me some solutions and suggestions? Thank you very much Best wishes, Clarence Hsiang |