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From: Lavanya R. <lav...@gm...> - 2016-02-06 12:55:19
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Hi, To start with, MUMmer is a great package! Thanks for having such a nice manual with it! I am using nucmer followed by mummerplot to draw alignment plots and I have a couple of questions regarding it. My commands are as follows: nucmer -p prefixA ref.fasta query.fasta mummerplot -p prefixB -png prefixA.delta This generates me a rather pretty looking plot which is great (please see the attached file). My questions are: 1) How big is the default window size for each plotted point? If there is a variation in the size, is there a way I can figure out what the size is? 2) I am getting a set of vertical lines in all my dot plot at the same loci against a specific reference that I have. This is possible if the window size is really small but then I should be seeing a lot of noise which is also not the case. Is my interpretation correct? If yes, how can I eliminate these vertical lines (in the sense of controlling the parameters with nucmer/mummerplot)? If no, any ideas of what this could be? Your time is much appreciated! Thanks, Lava |